About
This transgenic line expresses GFP in cranial motor neurons. It was made using a construct that fuses Islet-1 promotor/enhancer sequences to GFP.
More images of this transgenic
![isl1:GFP lateral 1dpf with ZO1(grey)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102431953-B8S5C9074EGM20OZDV12/isl1+lat+zo1+1dpf.png)
![isl1:GFP lateral 1dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102354408-VOC6MQ3FXF8FRWTSNFUE/isl1+1dpf+lat+2.png)
![isl1:GFP lateral 1dpf GFP and tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102354546-XMO8ZRA2XYME2MDHD22Q/isl1+1dpf+lat+GT.png)
![isl1:GFP lateral 1dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102357563-1VAXTB0NY422LV9KK7Z9/isl1+1dpf+lat.png)
![isl1:GFP lateral 1dpf with tubulin (cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102391077-6LJ2Q47GKL3KAMSC6NHY/isl1+lat+1dpf+GT.png)
![isl1 lateral 1dpf spinal cord](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102359637-PF5H4S5065CVR9P1EYCE/isl1+1dpf+spinal+cord.png)
![isl1:GFP ventral 36hpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102430712-ZKZGAUQ1Z0HU3B54LK8G/isl1+vent+36hpf.png)
![isl1 36h vent.png](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102367123-6B9T3TECXKKXEJABFQH5/isl1+36h+vent.png)
![isl1 dorsal 1dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102371660-R8ZAPR8T85PM581SH1RU/isl1+dorsal+1dpf.png)
![isl1:GFP 36hpf lateral with tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102422879-NITQ720HIICJ0GSN9XU5/isl1+lat+36hpf+GT.png)
![isl1:GFP 36hpf lateral](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102423454-3O8H9G0Y47LERW0Q4K20/isl1+lat+36hpf.png)
![isl1:GFP 60hpf dorsal with tubulin (cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102386727-TXBDBMV1Y1BBM843LVO8/isl1+dorsal+60hpf+GT.png)
![isl1:GFP 60hpf dorsal](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102386911-L8M9ZJ8L5HNNJG2HNABH/isl1+dorsal+60hpf.png)
![isl1:GFP 60hpf lateral with tubulin (cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102408085-U131SJWLLIL99478J0SD/isl1+lat+4dpf+eye+GT.png)
![isl1:GFP 60hpf lateral](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102405330-Y5WHTVCWMXBTKCFQLXEK/isl1+lat+4dpf+eye.png)
![isl1:GFP 60hpf ventral with tubulin (cyan](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102435332-HIMXOIQB7S1SNVIMPBC5/isl1+vent+60hpf+GT.png)
![isl1:GFP 60hpf ventral](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102435237-NE6FC4DOMOHFHVMCUJVD/isl1+vent+60hpf.png)
![isl1:GFP 3dpf dorsal with tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102377318-XRLX6O8SJ289M0B3ZQVU/isl1+dorsal+3dpf+GT.png)
![isl1:GFP 3dpf dorsal](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102377614-P8H9KOJZMXFN0X0X8CFR/isl1+dorsal+3dpf.png)
![isl1 lateral 3dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102390608-89AZ0AYKQ33A9TD5UV2U/isl1+lat+3dpf+2.png)
![isl1:GFP 3dpf lateral forebrain](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102399709-9Z44NI1WZCY7QPE6JRV1/isl1+lat+3dpf.png)
![isl1:GFP 3dpf lateral forebrain with tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102397085-EAI7T3G94T06YGNIRKX6/isl1+lat+3dpf+GT.png)
![isl1:GFP dorsal 4dpf with tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102365714-53WMG6109T3BASGHZ9R0/isl1+4dpf+dorsal+GT.png)
![isl1:GFP dorsal 4dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102382202-0MKUTNX3Y4NQ7IGXJCVJ/isl1+dorsal+4dpf.png)
![isl1:GFP lateral 4dpf with tubulin(cyan)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102366077-60KPE8S2GQCYSMGWVKNH/isl1+4dpf+lat+GT.png)
![isl1:GFP 4dpf lateral](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102408782-IXVIYNBIDIIKKMJPSH2G/isl1+lat+4dpf.png)
![isl1:GFP lateral 5dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102415392-JEZW0XBFORJK97H1C0PN/isl1+lat+5dpf.png)
![isl1:GFP 7dpf lateral forebrain with ZO1(grey)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102420579-BSLP4H1A04670IA1XTEK/isl1+lat+7dpf+zo1.png)
![isl1:GFP 7dpf lateral forebrain](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1553102428240-4EK6KS18XMUKREBEETTV/isl1+lat+forebrain+7dpf.png)
Expressed in:
cranial motor neurons, hindbrain, subpallium, preoptic area.
Key Publications
Higashijima, S., Hotta, Y., and Okamoto, H. (2000)
Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.
The Journal of neuroscience. 20(1):206-218
Suli, A., Mortimer, N., Shepherd, I., and Chien, C.B. (2006)
Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons.
The Journal of neuroscience. 26(51):13328-13337.
Schoppik, D., Bianco, I.H., Prober, D.A., Douglass, A.D., Robson, D.N., Li, J.M.B., Greenwood, J.S.F., Soucy, E., Engert, F., Schier, A.F. (2017)
Gaze-stabilizing central vestibular neurons project asymmetrically to extraocular motoneuron pools. The Journal of neuroscience. 37(47):11353-11365.
Rebman, J.K., Kirchoff, K.E., Walsh, G.S. (2016)
Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons.
PLoS One. 11:e0164433.
Barsh, G.R., Isabella, A.J., Moens, C.B. (2017)
Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation.
Current biology : CB. 27(24):3812-3825.e3.
Cox, J.A., Lamora, A., Johnson, S.L., and Voigt, M.M. (2011)
Diverse mechanisms for assembly of branchiomeric nerves.
Developmental Biology. 357(2):305-17.