About
Synonyms: Et(gata2a:EGFP)pku2, Et(gata2a:GFP)zf81, pku2Et, ETvmat2:GFP
The ETvmat2:GFP transgenic zebrafish line was identified from a large scale enhancer trap screen (unpublished data (Wen et al., 2008)) using a Tol2 vector containing a 249 bp zebrafish gata2 minimal promoter linked to a GFP reporter gene. (Wen et al., 2008). The enhancer trap vector was inserted in the second intron of vmat2 gene with the transcription direction of the GFP reporter opposite to that of the vmat2 gene (Wen et al., 2008).
This enhancer trap line recapitulates the expression pattern of vmat2 and labels most monaminergic neurons in the zebrafish. Vesicular monoamine transporter 2 (Vmat2) is a monoamine transporter VMAT2 is a membrane protein that transports monoamines, including neurotransmitters such as dopamine, norepinephrine, serotonin, and histamine from the cytosol into synaptic vesicles.
![4dpf Ventral view of ETvmat2:GFP.](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568210124944-MCFLVYIHT7UXE3GQJNNS/Screenshot+2019-09-11+14.53.56.png)
![4dpf Ventral view of ETvmat2:GFP with acetylated tubulin(magenta)..](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568210142094-6EQ1O5DZBOWAAEMRGNZS/Screenshot+2019-09-11+14.53.32.png)
![4dpf Lateral view of ETvmat2:GFP with acetylated tubulin(magenta).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568627686731-BWN41SM75E9X3GNZKME2/Screenshot+2019-09-16+10.50.13.png)
![4dpf Lateral view of ETvmat2:GFP](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568627684133-HQFZ7M3RM7O9CSS2Y5EP/Screenshot+2019-09-16+10.50.04.png)
![4dpf dorsal view of ETvmat2:GFP with acetylated tubulin(magenta).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568627748344-0PT5MXAQTAIDFS05X90V/Screenshot+2019-09-16+10.49.54.png)
![4dpf dorsal view of ETvmat2:GFP.](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568628784872-QJNZ4XQXX755E0TU117L/Screenshot+2019-09-16+10.49.44.png)
![5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631414473-7DETO1T4URJ1FK4MN99I/Snapshot+of+Series003+%28vmat_tub_sv2_4+tif%29_49.jpg)
![5dpf Ventral view of ETvmat2:GFP](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631414222-5C9788X3FVKNA13XMRVH/Snapshot+of+Series003+%28vmat_tub_sv2_4+tif%29_50.jpg)
![5dpf dorsal view of ETvmat2:GFP forbrain with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631416253-YINNA6FIM4NBN5BQTNBV/Snapshot+of+Series004+%28vmat_sv2_tub+5d_5%29_34.jpg)
![5dpf dorsal view of ETvmat2:GFP forebrain.](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631415991-IAQIJLDUZR1KMTTWZGU4/Snapshot+of+Series004+%28vmat_sv2_tub+5d_5%29_38.jpg)
![[Improvision Data]ImageName=TimeStampMicroSeconds=3319455431398154TimeStamp=14:57:11.398 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicro](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631946526-34AZDYGG28NB7Q4A9WXX/Snapshot+of+Series008+%28vmat_tub_sv2_5d%29_10.jpg)
![dpf dorsal view of ETvmat2:GFP hindbrain with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631418042-CEFXKTRSEH54VI6MC8CI/Snapshot+of+Series004+%28vmat_sv2_tub+tiff%29_39.jpg)
![[Improvision Data]ImageName=TimeStampMicroSeconds=3319455437197463TimeStamp=14:57:17.197 on 09 Mar 2009ChannelName=ChannelNo=1TimepointName=1TimepointNo=1ZPlane=1BlackPoint=0WhitePoint=255WhiteColour=255,255,255XCalibrationMicrons=1YCalibrationMicro](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631420219-DNOWMW8T30HIN5ZAE238/Snapshot+of+Series008+%28vmat_tub_sv2_5d%29_11.jpg)
![5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631946385-M4V6VGSSTV6SAEG4RZI5/Snapshot+of+Series003+%28vmat_tub_sv2%29_20.jpg)
![5dpf Frontal view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631947745-KJBTL052DBH4BMVICPHH/Snapshot+of+Series008+%28vmat_tub_sv2%29_21.jpg)
![5dpf dorsal view of ETvmat2:GFP hindbrain.](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631418429-SICBO6C0UJXKZCRVA5BB/Snapshot+of+Series004+%28vmat_sv2_tub+tiff%29_43.jpg)
![5dpf Ventral view of ETvmat2:GFP with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631419389-O3JGUHQGOVIXQ9VFAFC8/Snapshot+of+Series005+%28vmat_tub_sv2_5d%29_16.jpg)
![5dpf Ventral view of ETvmat2:GFP forebrain with acetylated tubulin(cyan).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631421058-T9QQFSU1ERWYVIMVN0LN/Snapshot+of+Series012+%28vmat_tub_sv2%29_25.jpg)
![5dpf Ventral view of ETvmat2:GFP forebrain.](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631422557-PW7POBM0U8J2RB6SO5EQ/Snapshot+of+Series012+%28vmat_tub_sv2%29_29.jpg)
![5dpf Lateral view of ETvmat2:GFP diencephalon with acetylated tubulin(cyan) and SV2(red).](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631424009-GYKDP7JPFFYXVM5DBAGP/Snapshot+of+Series014+%28vmat_tub_sv2_5d%29_12.jpg)
![5dpf Lateral view of ETvmat2:GFP diencephalon](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1568631424870-HA9SRJUW3UH5XF4M69UL/Snapshot+of+Series014+%28vmat_tub_sv2_5d%29_13.jpg)
Expressed in:
monoaminergic neurons, olfactory bulb, telencephalon, pretectum, pineal, posterior tuberculum, torus longitudinalis, raphe, ventral lateral group of serotinergic neurons in hindbrain(VL) , hypothalamus, locus coerulus.
Key Publications
Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B. (2008)
Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish. Developmental Biology. 314(1):84-92.
Farrar, M.J., Kolkman, K.E., Fetcho, J.R. (2018)
Features of the structure, development and activity of the Zebrafish Noradrenergic System explored in new CRISPR transgenic lines.
The Journal of comparative neurology. 526(15):2493-2508.