About
The hedgehog family of genes encodes a group of morphogenic preoteins that have a critical role in the development and patterning of midline brain structures and other systems. In zebrafish two of the three vertebrate hh genes are duplicated shha and shhb. This trangenic from the Nüsslein-Volhard lab has been used to study the role that Shh plays in patterning both the retina and the diencephalon.
All images of shh:GFP were produced by Monica Folgueira.
![4dpf lateral shh:GFP with acetylated tubulin(cyan) and sytox(grey)](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1567600877585-JZDR6L42L0LGWDWDRHUE/shh-02.png)
![shh-01.png](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1567600871214-UGMX18P9IW3C6AEWFZ20/shh-01.png)
![shh-03.png](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1567600874885-3JFRHRJ1K46CZ2Y0PUVY/shh-03.png)
![shh:GFP, tubulin and sytox 4dpf lateral forebrain](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886353781-LIOAP5695SRSI9TMRXB3/Snapshot+of+Series003+%28shh+tub+sytox+4.5d%29_02.jpg)
![shh:GFP and tubulin. 4 dpf lateral forebrain](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886355073-8J60ZB9HZORJG40A1PC9/Snapshot+of+Series006+%28shh+tub+sytox+4.5d%29_12.jpg)
![shh:GFP and tubulin. 4dpf dorsal view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886355069-XLU64XPRI678K83CX9H2/Snapshot+of+Series002+%28shh+4d%29_18.jpg)
![single z-slice through telencephalon](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886356185-DS5YAG1NYR6U14VG3BAA/Series003+%28shh+tub+sytox+4.5d%29+%28XY%29_03.jpg)
![shh:GFP dorsal view of 4dpf larvae](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886357224-CFYLIF4R1KIPIRAVE6SQ/Snapshot+of+Series002+%28shh+4d%29_19.jpg)
![Frontal transverse z-slice through the telencephalon of shh:GFP](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886357969-LW9NYYSRMMZG79IOSU90/Series016+%28shh+tub+sytox+4.5d%29+%28XY%29_08.jpg)
![shh:GFP and tubulin, 4dpf lateral view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886358935-565BQ5OYKSP98MWPEZGP/Snapshot+of+Series008+%28shh_tub+4dpf%29_04.jpg)
![shh:GFP lateral view 4dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886360584-U9FGNOQYYICRO3RU92BS/Snapshot+of+Series008+%28shh_tub+4dpf%29_03.jpg)
![shh:GFP, tubulin 4dpf ventral view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886359623-37G79YUSDTU9JC0XHCFH/Snapshot+of+Series002+%28shh+3d%29_25.jpg)
![shh:GFP and tubulin 24hpf lateral view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886361279-IO013KTDTEBL34VJQUNQ/Snapshot+of+Series009+%28shh+1d%29_26.jpg)
![shh:GFP lateral forebrain at 24hpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886361863-4HVKWAFVM9E0ZHY0E38U/Snapshot+of+Series003+%28shh+1d%29_28.jpg)
![shh:GFP and tubulin lateral view of 24hpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1549886358793-0UILB2X9J8OGZMTQJQZ0/Snapshot+of+Series003+%28shh+1d%29_27.jpg)
Expressed in:
basal plate midbrain, zona limitans intrathalamica, prethalamus, retina.
Key Publications
Neumann, C.J. and Nüsslein-Volhard, C. (2000) Patterning of the zebrafish retina by a wave of sonic hedgehog activity. Science (New York, N.Y.). 289(5487):2137-2139.
Scholpp, S., Foucher, I., Staudt, N., Peukert, D., Lumsden, A., and Houart, C. (2007) Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development (Cambridge, England). 134(17):3167-3176