Synaptic Vesicle 2 (SV2)
ABOUT THIS ANTIBODY
Synaptic Vesicle 2 labels synaptic neuropil.
We frequently use this antibody in conjunction with anti-acetylated tubulin (IgG2b, Sigma), which labels beautifully the axonal connections, to aid anatomical orientation in transgenic specimens. In addition to these antibodies being informative from a neuroanatomical perspective, they are also invaluable as a tool for anatomical localisation. These antibodies can be used as a framework to easily compare the expression patterns of different transgenic lines and locate GFP-positive structure in the context of the brain. Both antibodies are mouse monoclonals but can be detected in the same specimen using subtype-specific secondary antibodies.
Mouse monoclonal anti-SV2 (IgG1) (DSHB, Cat#AB 2315387, dilution 1:500)
Select images
![Telencephalic neuropil dorsal view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1518539371651-WJGK8TIPLP9OLGIRGVBZ/SV2_tub_dorsal_5d.jpg)
![Frontal view of telencephalic neuropil](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1518539371539-8K810RHZDEKFBJMZFDS8/SV2_tub_frontal_5d.jpg)
![Lateral view of telencephalic neuropil](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1518539372719-XCXJADJJJS0TOSWTVJSK/SV2_tub_lateral_5d.jpg)
![Sv2(magenta) and tubulin(green) lateral view at 4dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550501902784-DY6GQ0JD2H4WIJKYE1IM/Screenshot+2019-02-18+14.57.25.png)
![SV2 at 4dpf lateral view](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550501901424-N5GQ22N2YQDPVNNZ1Y18/Screenshot+2019-02-18+14.57.45.png)
![SV2 (magenta) and tubulin (green) lateral view of whole brain at 4dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550501924462-LZ51KPTTNLDS2SZV3K5Y/Screenshot+2019-02-18+14.56.12.png)
![SV2 lateral view of whole brain at 4dpf](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550501922247-W009SDGX7OFJN5PMSNJE/Screenshot+2019-02-18+14.57.11.png)
![Dorsal view of undissected 4dpf larvae](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550503088640-ZQKOLG1JRCI6B2JD9LBR/Screenshot+2019-02-18+15.15.33.png)
![Screenshot 2019-02-18 15.15.46.png](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550503065551-EIQBGQYTRN8P7H65W3ZJ/Screenshot+2019-02-18+15.15.46.png)
![4dpf whole brain dissected](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550503155578-1C9YXBOUGIER44KD5R2K/Screenshot+2019-02-18+15.14.50.png)
![4dpf whole brain dissected](https://images.squarespace-cdn.com/content/v1/58065fb61b631b37ff3ce66a/1550503134095-MNKOB87WD5MA3PUVZ6HM/Screenshot+2019-02-18+15.15.09.png)
External Links:
LABELS THESE BRAIN STRUCTURES:
Synaptic neuropil
KEY PUBLICATIONS
Buckley K, Kelly RB (1985)
Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells.
J Cell Biol 100:1284–1294
Hendricks M, Jesuthasan S (2007)
Asymmetric innervation of the habenula in zebrafish.
J Comp Neurol 502:611–619
Turner KJ1, Bracewell TG, Hawkins TA.
Anatomical dissection of zebrafish brain development.
Methods Mol Biol. 2014;1082:197-214. doi: 10.1007/978-1-62703-655-9_14.